A method for routine measurements of total sugar and starch content in woody plant tissues.
Identifieur interne : 004328 ( Main/Exploration ); précédent : 004327; suivant : 004329A method for routine measurements of total sugar and starch content in woody plant tissues.
Auteurs : Pak S. Chow [Canada] ; Simon M. Landh UsserSource :
- Tree physiology [ 0829-318X ] ; 2004.
Descripteurs français
- KwdFr :
- MESH :
- analyse : Amidon, Glucides.
- composition chimique : Arbres, Feuilles de plante, Picea, Pinus, Populus, Racines de plante, Tiges de plante.
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : Carbohydrates, Starch.
- chemistry : Picea, Pinus, Plant Leaves, Plant Roots, Plant Stems, Populus, Trees.
Abstract
Several extraction and measurement methods currently employed in the determination of total sugar and starch contents in plant tissues were investigated with the view to streamline the process of total sugar and starch determination. Depending on the type and source of tissue, total sugar and starch contents estimated from samples extracted with 80% hot ethanol were significantly greater than from samples extracted with a methanol:chloroform:water solution. The residual ethanol did not interfere with the sugar and starch determination, rendering the removal of ethanol from samples unnecessary. The use of phenol-sulfuric acid with a phenol concentration of 2% provided a relatively simple and reliable colorimetric method to quantify the total soluble-sugar concentration. Performing parallel sugar assays with and without phenol was more useful for accounting for the interfering effects of other substances present in plant tissue than using chloroform. For starch determination, an enzyme mixture of 1000 U alpha-amylase and 5 U amyloglucosidase digested starch in plant tissue samples more rapidly and completely than previously recommended enzyme doses. Dilute sulfuric acid (0.005 N) was less suitable for starch digestion than enzymatic hydrolysis because the acid also broke down structural carbohydrates, resulting in overestimates of starch content. After the enzymatic digestion of starch, the glucose hydrolyzate obtained was measured with a peroxidase-glucose oxidase/o-dianisidine reagent; absorbance being read at 525 nm after the addition of sulfuric acid. With the help of this series of studies, we developed a refined and shortened method suitable for the rapid measurement of total sugar and starch contents in woody plant tissues.
DOI: 10.1093/treephys/24.10.1129
PubMed: 15294759
Affiliations:
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Landh Usser</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2004">2004</date><idno type="RBID">pubmed:15294759</idno><idno type="pmid">15294759</idno><idno type="doi">10.1093/treephys/24.10.1129</idno><idno type="wicri:Area/Main/Corpus">004229</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">004229</idno><idno type="wicri:Area/Main/Curation">004229</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">004229</idno><idno type="wicri:Area/Main/Exploration">004229</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">A method for routine measurements of total sugar and starch content in woody plant tissues.</title><author><name sortKey="Chow, Pak S" sort="Chow, Pak S" uniqKey="Chow P" first="Pak S" last="Chow">Pak S. Chow</name><affiliation wicri:level="1"><nlm:affiliation>Centre for Enhanced Forest Management, Department of Renewable Resources, University of Alberta, Edmonton, AB, T6G 2E3, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Centre for Enhanced Forest Management, Department of Renewable Resources, University of Alberta, Edmonton, AB, T6G 2E3</wicri:regionArea><wicri:noRegion>T6G 2E3</wicri:noRegion></affiliation></author><author><name sortKey="Landh Usser, Simon M" sort="Landh Usser, Simon M" uniqKey="Landh Usser S" first="Simon M" last="Landh Usser">Simon M. Landh Usser</name></author></analytic><series><title level="j">Tree physiology</title><idno type="ISSN">0829-318X</idno><imprint><date when="2004" type="published">2004</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Carbohydrates (analysis)</term><term>Picea (chemistry)</term><term>Pinus (chemistry)</term><term>Plant Leaves (chemistry)</term><term>Plant Roots (chemistry)</term><term>Plant Stems (chemistry)</term><term>Populus (chemistry)</term><term>Starch (analysis)</term><term>Trees (chemistry)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Amidon (analyse)</term><term>Arbres (composition chimique)</term><term>Feuilles de plante (composition chimique)</term><term>Glucides (analyse)</term><term>Picea (composition chimique)</term><term>Pinus (composition chimique)</term><term>Populus (composition chimique)</term><term>Racines de plante (composition chimique)</term><term>Tiges de plante (composition chimique)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Carbohydrates</term><term>Starch</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Amidon</term><term>Glucides</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Picea</term><term>Pinus</term><term>Plant Leaves</term><term>Plant Roots</term><term>Plant Stems</term><term>Populus</term><term>Trees</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Arbres</term><term>Feuilles de plante</term><term>Picea</term><term>Pinus</term><term>Populus</term><term>Racines de plante</term><term>Tiges de plante</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Several extraction and measurement methods currently employed in the determination of total sugar and starch contents in plant tissues were investigated with the view to streamline the process of total sugar and starch determination. Depending on the type and source of tissue, total sugar and starch contents estimated from samples extracted with 80% hot ethanol were significantly greater than from samples extracted with a methanol:chloroform:water solution. The residual ethanol did not interfere with the sugar and starch determination, rendering the removal of ethanol from samples unnecessary. The use of phenol-sulfuric acid with a phenol concentration of 2% provided a relatively simple and reliable colorimetric method to quantify the total soluble-sugar concentration. Performing parallel sugar assays with and without phenol was more useful for accounting for the interfering effects of other substances present in plant tissue than using chloroform. For starch determination, an enzyme mixture of 1000 U alpha-amylase and 5 U amyloglucosidase digested starch in plant tissue samples more rapidly and completely than previously recommended enzyme doses. Dilute sulfuric acid (0.005 N) was less suitable for starch digestion than enzymatic hydrolysis because the acid also broke down structural carbohydrates, resulting in overestimates of starch content. After the enzymatic digestion of starch, the glucose hydrolyzate obtained was measured with a peroxidase-glucose oxidase/o-dianisidine reagent; absorbance being read at 525 nm after the addition of sulfuric acid. With the help of this series of studies, we developed a refined and shortened method suitable for the rapid measurement of total sugar and starch contents in woody plant tissues.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15294759</PMID><DateCompleted><Year>2006</Year><Month>09</Month><Day>11</Day></DateCompleted><DateRevised><Year>2019</Year><Month>05</Month><Day>13</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0829-318X</ISSN><JournalIssue CitedMedium="Print"><Volume>24</Volume><Issue>10</Issue><PubDate><Year>2004</Year><Month>Oct</Month></PubDate></JournalIssue><Title>Tree physiology</Title><ISOAbbreviation>Tree Physiol</ISOAbbreviation></Journal><ArticleTitle>A method for routine measurements of total sugar and starch content in woody plant tissues.</ArticleTitle><Pagination><MedlinePgn>1129-36</MedlinePgn></Pagination><Abstract><AbstractText>Several extraction and measurement methods currently employed in the determination of total sugar and starch contents in plant tissues were investigated with the view to streamline the process of total sugar and starch determination. Depending on the type and source of tissue, total sugar and starch contents estimated from samples extracted with 80% hot ethanol were significantly greater than from samples extracted with a methanol:chloroform:water solution. The residual ethanol did not interfere with the sugar and starch determination, rendering the removal of ethanol from samples unnecessary. The use of phenol-sulfuric acid with a phenol concentration of 2% provided a relatively simple and reliable colorimetric method to quantify the total soluble-sugar concentration. Performing parallel sugar assays with and without phenol was more useful for accounting for the interfering effects of other substances present in plant tissue than using chloroform. For starch determination, an enzyme mixture of 1000 U alpha-amylase and 5 U amyloglucosidase digested starch in plant tissue samples more rapidly and completely than previously recommended enzyme doses. Dilute sulfuric acid (0.005 N) was less suitable for starch digestion than enzymatic hydrolysis because the acid also broke down structural carbohydrates, resulting in overestimates of starch content. After the enzymatic digestion of starch, the glucose hydrolyzate obtained was measured with a peroxidase-glucose oxidase/o-dianisidine reagent; absorbance being read at 525 nm after the addition of sulfuric acid. With the help of this series of studies, we developed a refined and shortened method suitable for the rapid measurement of total sugar and starch contents in woody plant tissues.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Chow</LastName><ForeName>Pak S</ForeName><Initials>PS</Initials><AffiliationInfo><Affiliation>Centre for Enhanced Forest Management, Department of Renewable Resources, University of Alberta, Edmonton, AB, T6G 2E3, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Landhäusser</LastName><ForeName>Simon M</ForeName><Initials>SM</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Canada</Country><MedlineTA>Tree Physiol</MedlineTA><NlmUniqueID>100955338</NlmUniqueID><ISSNLinking>0829-318X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D002241">Carbohydrates</NameOfSubstance></Chemical><Chemical><RegistryNumber>9005-25-8</RegistryNumber><NameOfSubstance UI="D013213">Starch</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D002241" MajorTopicYN="N">Carbohydrates</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D028222" MajorTopicYN="N">Picea</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D028223" MajorTopicYN="N">Pinus</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018515" MajorTopicYN="N">Plant Leaves</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018517" MajorTopicYN="N">Plant Roots</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018547" MajorTopicYN="N">Plant Stems</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013213" MajorTopicYN="N">Starch</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014197" MajorTopicYN="N">Trees</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2004</Year><Month>8</Month><Day>6</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2006</Year><Month>9</Month><Day>12</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2004</Year><Month>8</Month><Day>6</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">15294759</ArticleId><ArticleId IdType="doi">10.1093/treephys/24.10.1129</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Canada</li></country></list><tree><noCountry><name sortKey="Landh Usser, Simon M" sort="Landh Usser, Simon M" uniqKey="Landh Usser S" first="Simon M" last="Landh Usser">Simon M. Landh Usser</name></noCountry><country name="Canada"><noRegion><name sortKey="Chow, Pak S" sort="Chow, Pak S" uniqKey="Chow P" first="Pak S" last="Chow">Pak S. Chow</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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